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对人肠道病毒EV71型2009年广州分离株Guangzhou09构建序列系统进化树并分析其重组特点。P1、P2和P3区种系发生进化树提示该分离株发生重组,相似性曲线以及bootscan进一步分析显示该分离株于非结构编码蛋白2B片段区(核苷酸位置4 027bp)处存在EV71亚型C4Shanghai-FJ713137与CVA409-HQ728260型间重组。该分离株与中国大陆目前优势株流行趋势一致,是广州首例发生型间重组且由C4亚型及CVA4型发生重组。
The phylogenetic tree of human enterovirus EV71 2009 Guangzhou isolate Guangzhou09 was constructed and its recombination characteristics were analyzed. Phylogenetic tree of P1, P2 and P3 phylogenetic tree prompted recombination of the isolates, similarity curves and bootscan further analysis showed that the isolates in non-structural coding protein 2B fragment region (nucleotide position 427bp) EV71 subtype C4Shanghai-FJ713137 and CVA409-HQ728260 type recombination between. This isolate is in line with the prevailing trend of dominant strains in mainland China and is the first case of inter-genotype recombination in Guangzhou and recombination by C4 subtype and CVA4 genotype.