以人脐动脉为材料，用贴块法在含２０％新生牛血清的ＤＭＥＭ中培养出细胞，经电镜和马氏染色鉴定，确认培养出的细胞为平滑肌细胞。平滑肌细胞传至第１９代仍保持“谷与峰”生长特征。该法取材独辟蹊蹊径，具有取材容易，操作简单，费用低廉的特点。为人动脉平滑肌细胞的培养提供了一种方便、实用的方法。 Using human umbilical artery as material, the cells were cultured in DMEM containing 20% ​​newborn calf serum by the patch method. The cells were identified as smooth muscle cells by electron microscopy and Markov staining. Smooth muscle cells to the 19th generation still maintain “valley and peak” growth characteristics. The method is unique and creative, with easy to draw, simple operation, low cost features. Human artery smooth muscle cell culture provides a convenient and practical method.