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目的检测喉鳞状细胞癌、癌旁切缘组织中p27和p21蛋白的表达情况及其与患者预后的关系。方法随机选取2004—2010年间唐山市协和医院85例喉鳞状细胞癌手术切除标本(阴性切缘),同期喉黏膜良性病变79例(慢性炎症12例,喉乳头状瘤67例)作为对照。免疫组化采用SP法。观察喉癌、癌旁组织及喉良性病变中的p27及p21蛋白的表达。收集临床资料并进行随访。结果在随访>2年的喉癌患者中,有14例局部复发(复发组),71例无复发(无复发组)。p27蛋白在喉癌、癌旁组织及喉良性病变中阳性率分别为43.5%、84.7%和89.9%,而p21蛋白阳性率分别为38.8%、21.2%和3.8%。喉癌复发组和无复发组中p27和p21阳性率分别为21.4%和47.9%、85.7%和29.6%,两者差异极显著(P<0.01)。结论 p27和p21蛋白在喉鳞状细胞癌的发生和发展中可能起重要作用;切缘组织中p27失活及p21过表达与喉癌的局部复发密切相关。
Objective To detect the expression of p27 and p21 proteins in the laryngeal squamous cell carcinoma and adjacent noncancerous tissues and their relationship with prognosis. Methods Totally 85 specimens of laryngeal squamous cell carcinoma (negative margin) in the Union Hospital of Tangshan City from 2004 to 2010 were randomly selected. 79 patients with benign laryngeal mucosal lesions (chronic inflammation in 12 and laryngeal papilloma in 67) were selected as controls. Immunohistochemistry SP method. To observe the expression of p27 and p21 in laryngeal cancer, paracancerous tissues and benign laryngeal lesions. Collect clinical data and follow up. Results Of the patients with laryngeal cancer who were followed up for 2 years, 14 had local recurrence (relapse group) and 71 had no recurrence (no recurrence group). The positive rate of p27 protein was 43.5%, 84.7% and 89.9% in laryngeal cancer tissues, paracancerous tissues and benign laryngeal lesions respectively, while the positive rates of p21 protein were 38.8%, 21.2% and 3.8% respectively. The positive rates of p27 and p21 in recurrence group and non - recurrence group were 21.4% and 47.9%, 85.7% and 29.6% respectively, the difference was significant (P <0.01). Conclusion The p27 and p21 proteins may play an important role in the occurrence and development of laryngeal squamous cell carcinoma. Inactivation of p27 and overexpression of p21 in the marginal tissues are closely related to the local recurrence of laryngeal carcinoma.