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为了研究二系杂交小麦HMW-GS类型与蛋白质含量、沉淀值、面团形成时间和衰弱角斜率等品质性状的关系,采用SDS-PAGE方法分析了杂交种的高分子量麦谷蛋白亚基(HMW-GS)的组成。结果表明,在试验材料中普遍存在有劣质亚基N和2+12,它们的频率分别是60.4%,83.0%。在HMW-GS与F1籽粒品质性状关系的研究中发现,各位点对蛋白质含量的贡献率是Glu-B1>Glu-A1>Glu-D1;对沉淀值贡献率是Glu-B1>Glu-D1>Glu-A1;对形成时间和衰落角斜率的贡献率是Glu-D1>Glu-B1>Glu-A1。总体来说,位点按对品质的贡献率大小依次为Glu-B1>Glu-D1>Glu-A1。就单个亚基而言Glu-D1位点,杂合2+12/5+10>纯合2+12;Glu-A1位点蛋白质含量、SDS沉淀值和形成时间均为N/N>N/1;在Glu-B1位点,蛋白质含量是7+8/17+18=7+9/17+18=7+8/7+9=7+8/7+8>7+9/7+9;沉淀值的大小依次为7+8/17+18≥7+9/17+18=7+8/7+9=7+8/7+8≥7+9/7+9;形成时间是7+8/17+18>7+9/17+18=7+8/7+9=7+8/7+8=7+9/7+9;衰弱角斜率大小是7+9/17+18≥7+8/17+18=7+9/7+9≥7+8/7+9=7+8/7+8。在不同亚基组合中,具有N/N,7+8/7+8,2+12/5+10亚基的品质最好。杂合位点5+10亚基对2+12亚基的品质具有补偿效应。
In order to study the relationship between HMW-GS type and quality traits, such as protein content, sedimentation value, dough forming time and decay angle, we analyzed the HMW-GS ) Of the composition. The results showed that there were ubiquitous inferior subunits N and 2 + 12 in the tested materials, their frequencies were 60.4% and 83.0% respectively. The results showed that the contribution rate of each site to protein content was Glu-B1> Glu-A1> Glu-D1, and the contribution rate to the sedimentation value was Glu-B1> Glu-D1> Glu-A1; the contribution to the slope of the formation time and decay angle is Glu-D1> Glu-B1> Glu-A1. Overall, the order of contribution to quality was Glu-B1> Glu-D1> Glu-A1. In the case of a single subunit, Glu-D1 locus and heterozygous 2 + 12/5 + 10> homozygote 2 + 12; Glu-A1 locus protein content, SDS sedimentation value and formation time were N / N> 1; at the Glu-B1 site, the protein content is 7 + 8/17 + 18 = 7 + 9/17 + 18 = 7 + 8/7 + 9 = 7 + 8/7 +8> 7 + 9/7 + 9; the size of the sedimentation value in turn was 7 + 8/17 + 18 ≧ 7 + 9/17 + 18 = 7 + 8/7 + 9 = 7 + 8/7 + 8 ≧ 7 + 9/7 + 9; Is 7 + 8/17 + 18> 7 + 9/17 + 18 = 7 + 8/7 + 9 = 7 + 8/7 + 8 = 7 + 9/7 + 9; the magnitude of the decay angle is 7 + 9 / 17 + 18 ≧ 7 + 8/17 + 18 = 7 + 9/7 + 9 ≧ 7 + 8/7 + 9 = 7 + 8/7 + 8. Among the different subunit combinations, the best quality is N / N, 7 + 8/7 + 8, 2 + 12/5 + 10 subunits. The heterozygous 5 + 10 subunit has a compensatory effect on the quality of 2 + 12 subunits.