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目的探讨线粒体丝氨酸蛋白酶(Omi/Htr A2)在甲状腺乳头状癌中的表达及其与临床病理的关系。方法采用免疫组织化学方法和蛋白印迹法检测120例甲状腺乳头状癌、40例结节性甲状腺肿及40例正常甲状腺组织中Omi/Htr A2的表达水平,并分析其与甲状腺乳头状癌的临床病理特征的关系。结果免疫组织化学结果表明,甲状腺乳头状癌组织中Omi/Htr A2蛋白阳性率(71.67%)较结节性甲状腺肿(30.00%)、正常甲状腺组织(32.50%)明显升高,差异有统计学意义(P<0.05)。蛋白印迹法检测结果表明,Omi/Htr A2蛋白在甲状腺乳头状癌组织中的表达量为(1.184±0.060),明显高于结节性甲状腺肿组织(0.599±0.096)和正常甲状腺组织(0.606±0.097),差异有统计学意义(P<0.05)。Omi/Htr A2蛋白的表达与甲状腺乳头状癌的包膜浸润、淋巴结转移、肿瘤临床分期及肿瘤分化有关(P<0.05)。结论 Omi/Htr A2蛋白的表达可能成为判断甲状腺乳头状癌恶化程度及预后的指标。
Objective To investigate the expression of mitochondrial serine protease (Omi / Htr A2) in papillary thyroid carcinoma and its relationship with clinical pathology. Methods Immunohistochemistry and Western blotting were used to detect the expression of Omi / Htr A2 in 120 cases of thyroid papillary carcinoma, 40 cases of nodular goiter and 40 cases of normal thyroid tissue. The clinical data of Omi / Htr A2 in thyroid papillary carcinoma Pathological characteristics of the relationship. Results Immunohistochemical results showed that the positive rate of Omi / Htr A2 protein in thyroid papillary carcinoma was significantly higher than that in nodular goiter (30.00%) and normal thyroid tissue (32.50%), the difference was statistically significant Significance (P <0.05). The results of Western blotting showed that the expression of Omi / Htr A2 protein in papillary thyroid carcinoma was (1.184 ± 0.060), which was significantly higher than that in nodular goiter (0.599 ± 0.096) and normal thyroid tissue (0.606 ± 0.097), the difference was statistically significant (P <0.05). The expression of Omi / Htr A2 protein was associated with the invasion of papillary thyroid carcinoma, lymph node metastasis, clinical stage and tumor differentiation (P <0.05). Conclusion The expression of Omi / Htr A2 protein may be an indicator of the degree and prognosis of papillary thyroid carcinoma.