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目的探讨急性脑梗死(ACI)患者血清神经元特异性烯醇化酶(NSE)、S100β蛋白及超敏C-反应蛋白(hs-CRP)水平变化与患者病情及预后的关系。方法采用双抗体夹心ELISA法对33例脑梗死患者及25例健康对照组的血清S100β蛋白水平进行测定;并用散射比浊法测定血清hs-CRP含量,NSE的测定采用电化学发光的方法。观察并比较ACI患者在不同病情、不同梗死面积时的血清S100β蛋白、NSE和hs-CRP含量变化,采用Pearson法对三者进行相关性分析。结果脑梗死组患者血清S100β蛋白[(0.025±0.017)μg/L、NSE(10.957±5.915)μg/L、hs-CRP(7.147±10.503)mg/L]水平均显著高于正常对照组[(0.017±0.009)μg/L、(9.972±3.188)μg/L、(2.679±1.678)mg/L]增高(均P<0.05),并且随着神经功能损伤程度的增高和梗死面积的增加,血清S100β蛋白、NSE和hs-CRP含量亦明显升;S100β蛋白、NSE和hs-CRP水平间呈正相关(P<0.01,P<0.05)。结论急性脑梗死(ACI)患者血清S100β蛋白、NSE、hs-CRP水平可作为急性脑梗死患者病情判断、预后评估的指标;尤其适用于无法进行影像学检查的急性脑梗死患者。多指标相结合进行评价意义更大。
Objective To investigate the changes of serum neuron specific enolase (NSE), S100β protein and hs-CRP levels in patients with acute cerebral infarction (ACI) and their relationship with the disease status and prognosis. Methods Serum levels of S100βin 33 patients with cerebral infarction and 25 healthy controls were measured by double antibody sandwich ELISA. The serum hs-CRP levels were determined by nephelometry. The chemiluminescence was used to determine the NSE. The levels of serum S100β protein, NSE and hs-CRP in patients with ACI were observed and compared in different conditions and different infarct sizes. Pearson correlation was used to analyze the correlation between them. Results The levels of S100β protein [(0.025 ± 0.017) μg / L and NSE (10.957 ± 5.915) μg / L and 7.17 ± 10.503 mg / L] in patients with cerebral infarction were significantly higher than those in the normal control group [ 0.017 ± 0.009) μg / L, (9.972 ± 3.188) μg / L and (2.679 ± 1.678) mg / L], respectively (all P <0.05). With the increase of neurological injury and infarction area, The levels of S100β protein, NSE and hs-CRP also increased significantly. The levels of S100β protein, NSE and hs-CRP were positively correlated (P <0.01, P <0.05). Conclusions Serum levels of S100β protein, NSE and hs-CRP in patients with acute cerebral infarction (ACI) can be used as indicators of disease progression and prognosis in patients with acute cerebral infarction. It is especially suitable for patients with acute cerebral infarction who can not perform imaging examination. Multiple indicators combined to evaluate more meaningful.