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目的 研究不同分子量聚乙二醇 [poly(ethyleneglycol) ,PEG]表面修饰的硬脂酸脂质纳米粒的制备方法及体外细胞摄取的情况。方法 以亲脂端为硬脂基 ,亲水端为不同链长度PEG的非离子性表面活性剂 ,用“乳化蒸发 低温固化”的方法制备硬脂酸纳米粒。以小鼠腹腔巨噬细胞为细胞模型做体外细胞吞噬实验。结果 用Brij 78,Myrj5 3和Myrj5 9为表面活性剂制备了粒径分别为 (16 2 0± 6 7 4)nm ,(5 0 2± 2 8 9)nm和 (32 6 8± 195 2 )nm的纳米粒。体外细胞摄取实验证明 ,各种纳米粒相对于硬脂酸溶液均可增加巨噬细胞对硬脂酸的摄取 ,其中以Myrj5 9组摄取最少 ;在样品中加入小鼠血浆可以增加巨噬细胞对硬脂酸纳米粒的摄取。结论 用“乳化蒸发 低温固化”的方法可以制备PEG表面修饰的硬脂酸纳米粒 ;表面修饰PEG链的长度可以影响硬脂酸纳米粒体外细胞的摄取。
Aim To study the preparation method and in vitro cell uptake of stearic acid lipid nanoparticles with different molecular weights on poly (ethyleneglycol) (PEG) surface modification. Methods Stearic acid nanoparticles were prepared by emulsification and evaporation at low temperature using nonionic surfactants with lipophilic end as stearyl group and hydrophilic end with different chain length PEG. Mouse peritoneal macrophages as a cell model in vitro phagocytosis experiments. Results The nanoparticles with diameters of (16 2 0 ± 6 7 4) nm, (5 0 2 ± 2 8 9) nm and (32 6 8 ± 195 2) were prepared with Brij 78, Myrj 5 3 and Myrj 5 9 as surfactants, nm nanoparticles. In vitro cell uptake experiments showed that various nanoparticles can increase stearic acid uptake by macrophages relative to stearic acid solution, with the lowest in Myrj5 9 group; the addition of mouse plasma to the sample can increase the macrophage pairs Stearic acid nanoparticles uptake. Conclusion The PEG surface modified stearic acid nanoparticles can be prepared by the method of “emulsion curing at low temperature”. The length of surface modified PEG chains can affect the in vitro cell uptake of stearic acid nanoparticles.