论文部分内容阅读
观察环孢霉素A(CsA)对羟基喜树碱 (HCPT)、三尖杉酯碱 (HT)诱导白血病细胞凋亡的调节作用。方法 :应用细胞形态学检查 ,DNA凝胶电泳及流式细胞术分析检测。结果 :CsA3μg/ml对HL 60细胞生长无明显影响 ,也没有促进凋亡作用。HL 60细胞加上HCPT0 5μg/ml、HT0 1μg/ml作用4h ,细胞凋亡率分别为(35 7±1 3) %和(31 9±0 8) %。先以CsA3μg/ml作用HL 60细胞24h ,洗涤 ,然后再加HCPT0 5μg/ml、HT0 1μg/ml继续作用4h ,细胞凋亡率明显增加 ,达(47 5±0 5) % (P<0 01)和(44 3±0 8) % (P<0 01)。CsA +HCPT及CsA +HT组分别比单独HCPT、HT组诱导的DNA梯状条带亮度增强。CsA3μg/ml对HL 60细胞的bcl 2蛋白表达以及周期分布均无影响。结论 :CsA能提高HL 60细胞对HCPT或HT诱导凋亡敏感性。提示临床上应用CsA联合HCPT或HT治疗白血病 ,可能取得较好疗效
The effect of cyclosporine A (CsA) on the apoptosis of leukemia cells induced by HCPT and harringtonine (HT) was observed. Methods : Cell morphology examination, DNA gel electrophoresis and flow cytometry analysis were used. RESULTS: CsA 3μg/ml had no significant effect on the growth of HL 60 cells and did not promote apoptosis. When HL 60 cells were treated with HCPT0 5 μg/ml and HT0 1 μg/ml for 4 h, the apoptosis rate was (35 7±1 3) % and (31 9±0 8) %, respectively. The HL60 cells were treated with CsA3μg/ml for 24h, washed, and then treated with HCPT0 5μg/ml and HT0 1μg/ml for 4h, and the apoptosis rate increased significantly (47 5±0 5) % (P<0 01). ) and (44 3±0 8) % (P<0 01). CsA + HCPT and CsA + HT groups had stronger DNA laddering bands than HCPT and HT groups, respectively. CsA3μg/ml had no effect on the expression of bcl 2 protein and the cycle distribution of HL 60 cells. Conclusion: CsA can increase the sensitivity of HL 60 cells to apoptosis induced by HCPT or HT. Prompt clinical application of CsA combined with HCPT or HT in the treatment of leukemia may achieve better results