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用细胞分离、免疫组织化学及同位素标记方法研究大鼠精子细胞变态期核蛋白转换（ＨＰＲＲ）所取得的结果。用速度沉降法（ｓｔａｐｕｔｍｅｔｈｏｄ）及超声法得到高纯度的睾丸圆形、长形精子细胞核及附睾精子核，提取其总碱性核蛋白（ＴＮＢＰ），电泳分析，发现睾丸圆形精子细胞核内为组蛋白；长形精子细胞核内主要为精核蛋白、少量过渡形蛋白（ＴＰｓ）及组蛋白；附睾精子核中主要为精核蛋白。免疫组织化学研究表明，只有长形精子细胞核内检测到精核蛋白，而圆形精子细胞、精母细胞及精原细胞核均为免疫组织化学阴性反应。用１４Ｃ－精氨酸注入大鼠睾丸后，分离睾丸圆形及长形精子细胞核，提取碱性核蛋白电泳后，测定各蛋白条带的放射量。在长形精子细胞检测到大量放射性精核蛋白存在，表明精核蛋白的生物合成及积聚是在此细胞阶段。上述研究结果表明，在大鼠精子形成中，其核蛋白经历了由组蛋白到精核蛋白的转换过程，精核蛋白是成熟精子的主要核蛋白。本文还对ＨＰＲＲ是精子发生中的薄弱环节和借此进行生育调控的可能性予以讨论。
Cell isolation, immunohistochemistry and isotope labeling were used to investigate the results of rat nuclear translocation (HPRR) during the metamorphosis. High-purity testicular round and spermatozoon nuclei and epididymal sperm nuclei were obtained by staplermethod and sonication. The total alkaline nucleoprotein (TNBP) was extracted and analyzed by electrophoresis. The main nuclei of spermatozoa were spermatogonia, a few transitional proteins (TPs) and histones. The main nuclei of the epididymal sperm nucleus were nucleoprotein. Immunohistochemical studies showed that only spermatogonia were detected in the nuclei of spermatozoa, whereas round spermatids, spermatocytes and spermatogonia were all negative for immunohistochemistry. After injection of 14C-arginine into rat testis, the nuclei of round and elongated spermatozoa were separated from the testis, and the radioactivity of each protein band was determined after electrophoresis of the alkaline nucleoprotein was extracted. The presence of a large number of radionuclides was detected in spermatogenic cells, indicating that the biosynthesis and accumulation of protamine is at this cellular stage. The above results show that in rat spermatogenesis, the nucleoprotein undergoes the conversion from histone to protion, which is the major nucleoprotein of mature spermatozoa. This article also discusses the possibility that HPRR is a weak link in spermatogenesis and is responsible for fertility control.