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目的观察二硫代氨基甲酸吡咯烷(PDTC)对重症急性胰腺炎肺损伤(PALI)的保护作用。方法 SD大鼠60只,分为对照组、重症急性胰腺炎(SAP)组、PDTC预处理组,每组再分为3h、6h、9h及12h四个时间组。采用5%牛磺胆酸钠胰胆管逆行注射诱发大鼠SAP模型。PDTC预处理组在建立SAP模型前1h腹腔内注射PDTC。检测血清淀粉酶并通过透射电镜观察肺组织超微结构改变。结果透射电镜下SAP各组可见Ⅱ型肺泡上皮细胞线粒体肿胀、核浓缩等现象。PDTC预处理组与相对应的SAP各组比较均有不同程度减轻。结论 PDTC预处理各组大鼠胰、肺组织病理学改变减轻,提示PDTC对SAP肺损伤具有保护作用。
Objective To observe the protective effect of pyrrolidine dithiocarbamate (PDTC) on lung injury of severe acute pancreatitis (PALI). Methods Sixty Sprague-Dawley rats were randomly divided into four groups: control group, severe acute pancreatitis (SAP) group and PDTC preconditioning group. Each group was further divided into 4 groups: 3h, 6h, 9h and 12h. The rat SAP model was induced by retrograde injection of 5% sodium taurocholate and pancreaticobiliary duct. The PDTC pretreatment group was intraperitoneally injected with PDTC 1 h before SAP model was established. Serum amylase was detected and the ultrastructure of lung tissue was observed by transmission electron microscope. Results Under the transmission electron microscope, the mitochondrial swelling and nuclear condensation of type Ⅱ alveolar epithelial cells were observed in all the groups of SAP. PDTC pretreatment group and the corresponding SAP groups were all reduced to varying degrees. Conclusion PDTC pretreatment of rat pancreas, lung pathological changes alleviated, suggesting that PDTC has a protective effect on SAP lung injury.