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目的探讨趋化因子T细胞激活分泌调节因子(RANTES)对肥大细胞Toll样受体4(TLR4)表达的调节作用。方法将小鼠肥大细胞P815分为:RANTES 0.1、1.0、10、100ng/ml处理组(分别为A1、A2、A3、A4组)、RANTES 100ng/ml+RANTES阻断抗体10、30μg/ml组(分别为B1、B2组)以及空白对照组(C组)。作用2、6、16h后,采用流式细胞术、免疫荧光、RT-PCR检测肥大细胞上TLR4的表达情况。结果与C组相比,RANTES呈剂量依赖性地上调P815细胞中TLR4mRNA和蛋白表达(P<0.05),而B1、B2组能明显抑制该上调过程(P<0.05)。结论 RANTES上调P815细胞中TLR4的表达,从而加重过敏反应。
Objective To investigate the regulatory effect of RANTES on the expression of Toll-like receptor 4 (TLR4) in mast cells. Methods The mouse mast cells P815 were divided into three groups: RANTES 0.1, 1.0, 10, 100ng / ml (A1, A2, A3 and A4), RANTES 100ng / ml + RANTES blocking antibody 10 and 30μg / ml (B1 and B2 groups, respectively) and blank control group (C group). After 2, 6 and 16 hours, the expression of TLR4 on mast cells was detected by flow cytometry, immunofluorescence and RT-PCR. Results Compared with group C, RANTES up-regulated the mRNA and protein expression of TLR4 in P815 cells in a dose-dependent manner (P <0.05), while B1 and B2 inhibited the up-regulation of TLR4 mRNA and protein (P <0.05). Conclusion RANTES up-regulates the expression of TLR4 in P815 cells, which aggravates the allergic reaction.