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采用InertsilODS色谱柱(46mm×250mm),以甲醇水(15∶85)为流动相,280nm为检测波长,用RPHPLC法测定了越桔茎叶中熊果甙的平均含量为444%,RSD为293%;平均加样回收率为1007%,RSD为285%。
Using Inertsil-ODS column (46mm×250mm), methanol-water (15∶85) as the mobile phase and 280nm as the detection wavelength, the average content of bearberry pods in leaves and stems of Bilberry was determined by RPHPLC method. 44%, RSD was 293%; average sample recovery was 1007%, RSD was 285%.