论文部分内容阅读
目的通过向10d龄乳鼠缺血缺氧模型注入USPIO标记人脐带间充质干细胞(MSCs),利用3.0TMR监测MSCs进入鼠体内后的生存、转化、迁移,探索MSCs移植活体示踪技术;观察MRI图像变化及神经学评分,初步探讨MSCs对乳鼠缺氧缺血性脑病(HIE)的治疗效果,并分析其治疗机制。方法培养MSCs,以多聚赖氨酸(PLL)为转染剂,用USPIO对MSCs-PLL进行磁性标记;将磁标记的MSCs通过鼠尾静脉移植入HIE乳鼠体内,分别于移植前、移植后1d,10d行MRI观察及神经学评分(NSS评分)。结果 1USPIO-PLL可安全有效标记MSCs,标记率大于96%,细胞活性无影响;2移植后1d MRI可监测到MSCs到达病变区,10d时到达病变区MSCs增多,MRI信号降低(60.66±15.28)%(P<0.05);建模成功后,MSCs组移植后1d、移植后10d脑梗死体积相对体积组间差异有统计学意义(P<0.05);NS组(生理盐水对照组)差异无统计学意义(P>0.05);MSCs组与NS组移植10d脑梗死体积相对体积的变化值比值差异有统计学意义(P<0.05)3MSCs移植组、NS组移植10d后及移植前神经病学评分分别为(3.2±2.1),(8.0±1.9),(8.1±1.8)分,组间差异有统计学意义(P<0.05)。结论 MRI可以有效进行干细胞移植后的活体示踪;干细胞对缺血缺氧梗死区有定向趋化作用,可以促进受损神经恢复而起到治疗作用;为新生儿HIE临床治疗提供了有力的实验依据。
OBJECTIVE: To detect the survival, transformation and migration of MSCs into MSCs by using USPIO-labeled human umbilical cord mesenchymal stem cells (MSCs) injected into 10-day-old suckling mice hypoxic-ischemic model by 3.0TMR. MRI image changes and neurological score, preliminary study of MSCs on neonatal hypoxic-ischemic encephalopathy (HIE) treatment effect, and analysis of its therapeutic mechanism. METHODS: MSCs were cultured and poly-L-lysine (PLL) was used as a transfection reagent. MSCs-PLL was magnetically labeled with USPIO. Magnetic labeled MSCs were transplanted into the HIE mice via tail vein of rats. MRI and neurological score (NSS score) were performed on the 1st and 10th postoperative day. Results 1USPIO-PLL could label MSCs safely and effectively with a labeling efficiency of more than 96% and had no effect on cell viability. 2 MSCs reached the diseased zone on the 1st day after transplantation, and reached the lesion site on the 10th day after transplantation. MRI signals decreased (60.66 ± 15.28) % (P <0.05). After successful modeling, there was a significant difference in the relative volume of cerebral infarction between the MSCs group at 1 day and 10 days after transplantation (P <0.05); there was no statistical difference in the NS group (NS control group) (P> 0.05). There was significant difference in the relative volume of infarct volume between MSCs group and NS group 10 days after transplantation (P <0.05). The neurological score of MSCs transplantation group and NS group 10 days after transplantation and pre-transplantation neurological score (3.2 ± 2.1), (8.0 ± 1.9), (8.1 ± 1.8) points, the difference between the two groups was statistically significant (P <0.05). Conclusion MRI can be used to perform in vivo tracing after stem cell transplantation. Stem cells can induce chemotaxis in ischemic infarct area and promote the recovery of injured nerve. It provides a powerful experimental method for clinical treatment of neonatal HIE in accordance with.