论文部分内容阅读
目的 建立一种简便快速、经济实用的胆囊棘球蚴病 (cysticechinococcosis ,CE)的实验诊断方法。方法 以自制兔抗棘球蚴抗血清建立一种检测CE患者血清或尿液中棘球蚴抗原 (H -Ag)的玻片协同凝集 (Co -A)试验 ,并用于检测 30例CE患者、2 0例其他寄生虫病和 2 5例健康人血清及尿液中的H -Ag ,并评价该法的灵敏度、特异度、阳性预测值和阴性预测值。结果 CE患者浓缩尿液和血清中H -Ag的总检出率分别为 77%及 83 % ,其中手术诊断CE组浓缩尿液和血清中H -Ag的检出率分别为 83 %及 92 % ,超声检查证实CE组为 75 %及 88% ,临床诊断CE为 70 %及 80 %。应用Co -A试验检测患者浓缩尿液和血清中H -Ag的灵敏度、特异度、阳性预测值和阴性预测值分别为 77% ,87% ,93 %和 96 % ;89% ,93 % ,86 %和 92 %。结论 Co -A试验是临床上诊断CE的一种简便快速、经济实用的实验诊断方法 ,易于推广使用
Objective To establish a simple, rapid and economical method for the experimental diagnosis of cysticechococcosis (CE). Methods A self-made anti-Echinococcus anti-sera was used to establish a co-agglutination test (Co-A) in serum or urine of patients with CE. The co-agglutination test was performed on 30 patients with CE, 20 cases of other parasitic diseases and 25 cases of healthy human serum and urine H-Ag, and evaluation of the sensitivity, specificity, positive predictive value and negative predictive value. Results The total detection rate of H-Ag in concentrated urine and serum of patients with CE was 77% and 83% respectively. The detection rates of concentrated urine and serum H-Ag in CE were 83% and 92% respectively Ultrasound examination confirmed that the CE group was 75% and 88%, the clinical diagnosis of CE was 70% and 80%. The sensitivity, specificity, positive predictive value, and negative predictive value of Co-A test were 77%, 87%, 93% and 96%, respectively; 89%, 93% and 86% % And 92%. Conclusion Co-A test is a simple, rapid, economical and practical method of laboratory diagnosis of CE, which is easy to popularize