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采用ＤＮＡ末端转移酶介导的Ｂｉｏ－ｄＵＴＰ原位末端标记技术（ＴＵＮＥＬ）和免疫组织化学技术，分别对６０例胃癌标本的细胞凋亡和ｐ５３蛋白异常作同步研究和相关性分析。结果在６０例胃癌组织中细胞凋亡指数为１．８％～１９．４％（平均９．３±３．９％），ｐ５３蛋白异常表达率为６３．３％（３８／６０）；ｐ５３蛋白阳性组细胞凋亡指数为３．７±１．１％（１．８％～７．９％），而ｐ５３蛋白阴性组细胞凋亡指数１４．７±２．４％（７．４％～１９．４％），两组间有显著性差异。结果表明胃癌中存在较高频率的ｐ５３蛋白异常和细胞凋亡，ｐ５３蛋白异常与胃癌细胞凋亡间存在着负相关性
Using DNA-terminal transferase mediated TUNEL and immunohistochemical techniques, the apoptosis and p53 protein abnormalities were simultaneously studied and correlated in 60 gastric cancer specimens. Results The apoptotic index was 1.8% to 19.4% (mean 9.3±3.9%) in 60 cases of gastric cancer and 63.3% (38/60) in p53 protein; p53 The apoptosis index was 3.7±1.1% (1.8% to 7.9%) in the protein positive group and 14.7±2.4% (7.4%) in the p53 protein negative group. ~ 19.4%), there was a significant difference between the two groups. The results showed that there was a higher frequency of p53 protein abnormalities and apoptosis in gastric cancer, and there was a negative correlation between abnormal p53 protein and apoptosis of gastric cancer cells.