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目的研究医院环境表面分离的致病菌对常用消毒剂抗性变化情况,为科学使用化学消毒剂提供参考。方法采用稀释法和基因扩增技术,对医院环境中分离的致病菌抗消毒剂情况和抗性基因携带率进行检测与分析。结果苯扎溴铵对铜绿假单胞菌中的3株MIC和MBC值低于标准菌株;对3株肺炎克雷伯菌和鲍曼不动杆菌的MIC和MBC值高于标准菌株。葡萄糖酸氯己定对铜绿假单胞菌的MIC值和MIC值有2株高于标准株,有9株低于标准株;对肺炎克雷伯和鲍曼不动杆菌的MIC值和MBC值均与标准菌株相同。二氧化氯对铜绿假单胞菌中的MIC和MBC值有6株高于标准株,有2株低于标准株;对肺炎克雷伯菌的MIC和MBC值均低于标准菌株,对鲍曼不动杆菌的MIC、MBC值均高于标准菌株。医院分离的铜绿假单胞菌检出qac E△1-sul 1基因携带率为79.17%,其他两种致病菌均未检出携带抗性基因。结论医院物体表面分离的致病菌中仅少部分菌株对3种消毒剂产生了抗性,多数则比较敏感,对医院环境病原菌抗消毒剂情况予以关注非常必要。
Objective To study the changes of resistance to common disinfectants by pathogens isolated on the surface of hospital environment and to provide a reference for the scientific use of chemical disinfectants. Methods Dilution method and gene amplification technique were used to detect and analyze the antimicrobial agent and resistance gene carrier rate of pathogenic bacteria isolated in hospital environment. Results The MIC and MBC values of benzalkonium bromide against Pseudomonas aeruginosa were lower than those of the standard strains. The MIC and MBC values of three strains of Klebsiella pneumonia and Acinetobacter baumannii were higher than those of the standard strains. MIC value and MIC value of chlorhexidine gluconate against Pseudomonas aeruginosa were higher than those of standard strains and 9 strains were lower than that of standard strains. MIC value and MBC value of Klebsiella pneumoniae and Acinetobacter baumannii Same as standard strain. Chlorine dioxide on the Pseudomonas aeruginosa MIC and MBC value of 6 strains higher than the standard strain, 2 strains lower than the standard strain; Klebsiella pneumoniae MIC and MBC values were lower than the standard strain, the abalone The MIC and MBC values of A.niger were higher than those of the standard strains. Pseudomonas aeruginosa isolated from the hospital detected qac E △ 1-sul 1 gene carrier rate was 79.17%, the other two pathogens were not detected carrying resistance genes. Conclusion Only a few of the pathogenic bacteria isolated from the surface of hospital objects are resistant to three kinds of disinfectants, while most are more sensitive. It is necessary to pay attention to the anti-disinfectants of environmental pathogens in hospitals.