Objective:To investigate the inhibition effect of siRNA interference onNGF induced by inflammatory factorIL-6, andIL-1 so as to provide novel targets for clinical treatment of discogenic low back pain.Methods:The intervertebral disc nucleus and annulus fibrosus cells of rats were separated .The cells were co-cultured with different concentrations(10 nmol/L,20 nmol/L,50 nmol/L,100 nmol/L) ofIL-6 andIL-1β.TheNGF- siRNA was leaded into the co-cultured cells with its import ability assessed by flow cytometry instrument tests,before and after which theNGF mRNA expression was detected by real-timeQ-PCR and theNGF content was detected byELISA.Results:Flow cytometry instrument test results showed that theNGF-siRNA cell conversion rate was99.8%.Real-timeQ-PCR detection results showed that compared with negative control group, theNGF mRNA expression of co-cultured cells treated by10 nmol/L,20 nmol/L,50 nmol/L,100 nmol/LIL-6 andIL-1β were respectively raised3.4,3.7,4.7, 3.7 times which were all significantly down-regulated after the import ofNGF- siRNA.EILSA detection results showed that compared with negative control group, theNGF content of co-cultured medium treated by10 nmol/L,20 nmol/L,50 nmol/L,100 nmol/LI-L6 andIL-1β were respectively raised2.9,3.3,4.5,7.4 times which were all significantly decreased after the import ofNGF- siRNA.Conclusions:These molecular biological results suggest that inflammatory factorIL-6 andIL-1β could stimulateNGF on intervertebral disc cells in vitro culture model and its efficiency is concentration dependent, while siRNA interference can inhibit the stimulation effect ofIL-6 andIL-1β on intervertebral disc cell, which provides a new targets for the clinical treatment of discogenic low back pain.