论文部分内容阅读
目的：通过对血源性乙肝疫苗接种７年后的抽查，深入了解血源性疫苗的远期免疫效果，藉以评价血源性疫苗在阻断肝癌发生中的作用。方法：应用血源性乙肝疫苗对肝癌高发区６３２例血清乙肝二对半标志物全部阴性的０～７岁婴儿进行免疫接种。用ＥＬＩＳＡ及ＰＣＲ法分别检测随机抽查的３００例血清中的乙肝病毒二对半标志物及ＨＢＶＤＮＡ，并进行ＡＦＰ及肝功能检测。结果：ＨＢｓＡｇ阳性率为６．０％（１８／３００），其中仅有４４．４％（８／１８）应用ＰＣＲ方法检测到ＨＢＶＤＮＡ，ＨＢＶＤＮＡ阳性仅占全部抽检对象的２．７％（８／３００）。抗－ＨＢｓ的阳性率为７０．７％（２１２／３００）、ＨＢｓＡｇ的检出率、ＨＢＶＤＮＡ的阳性率均显著低于当地一般人群（Ｐ＜０．００１），而抗－ＨＢｓ转阳率亦显著高于同一人群的阳性率。结论：血源性乙肝疫苗具有很好的免疫效果，可有效地阻断乙肝病毒的水平传播。在ＨＢｓＡｇ阳性者中仅有少数能检测到ＨＢＶＤＮＡ，提示血源性乙肝疫苗可能有免疫调节作用，从而抑制ＨＢＶ的体内复制。
OBJECTIVE: To understand the effect of long-term immunization of blood-borne vaccines through random sampling seven years after inoculation of blood-borne hepatitis B vaccines to evaluate the role of blood-borne vaccines in blocking the occurrence of liver cancer. Methods: Blood-borne hepatitis B vaccine was used to immunize 0 to 7-year-old infants who were all negative for the two pairs of half markers of serum HBV in 632 high-risk areas of HCC. Two hundred and seventy-two markers of hepatitis B virus and HBVDNA in 300 randomly selected serum samples were detected by ELISA and PCR. AFP and liver function were detected. Results: The positive rate of HBsAg was 6.0% (18/300), only 44.4% (8/18) of them were positive for HBV DNA by PCR. The positive rate of HBVDNA was only 2.7% (8 / 300). The positive rate of anti-HBs was 70.7% (212/300). The positive rates of HBsAg and HBVDNA were significantly lower than those in the general population (P <0.001), while the positive rate of anti-HBs Significantly higher than the same population positive rate. Conclusion: Hematological hepatitis B vaccine has a good immune effect, which can effectively block the level of hepatitis B virus transmission. Only a few HBsAg-positive patients can detect HBVDNA, suggesting that hematological hepatitis B vaccine may have immunomodulatory effects, thereby inhibiting HBV in vivo replication.