论文部分内容阅读
用聚丙烯酰胺垂直平板电泳技术研究了不同底物（α－萘醋酸酯和β－萘醋酸脂）、不同偶联剂（固蓝ＲＲ盐和固蓝Ｂ盐）、ｐＨ及温度条件对大麦酯酶同工酶电泳显色的影响。结果为：（１）绝大多数酶带可用α－萘醋酸酯或β－萘醋酸酯显色，极少数酶带只能用α－萘醋酸酯显示，混合底物显色时，酶带数目与α－萘醋酸酯底物显色相同，只是酶带颜色有差异；（２）用固蓝ＲＲ盐作偶联剂染色与用固蓝Ｂ盐相比，多数酶带的相对染色强度提高；（３）ｐＨ６．４比较适合酯酶同工酶染色，且以３７℃１０ｍｉｎ为最佳。表明各电泳酶带染色强度的相对差异并不一定反映各同工酶本身含量、酶活性的相对高低。
Polyacrylamide vertical plate electrophoresis technique was used to study the effects of different substrates (α-naphthalene acetate and β-naphthalene acetate), different coupling agents (solid blue RR salt and solid blue salt B), pH and temperature conditions on barley ester Effect of Enzymatic Isozyme Electrophoresis. The results are as follows: (1) Most of the enzyme bands can be developed with α-naphthalene acetate or β-naphthalene acetate. Only a few of the enzyme bands can only be displayed with α-naphthalene acetate. When the mixed substrate is colored, the number of enzyme bands The same color as the α-naphthalene acetate substrate, except that the color of the enzyme band was different. (2) Compared with the solid blue B salt, the relative staining intensity of most of the enzyme bands increased with the use of the solid blue RR salt as the coupling agent. (3) pH6.4 more suitable for esterase isozyme staining, and 37 ℃ 10min for the best. Show that the relative differences between the electrophoresis band staining intensity does not necessarily reflect the content of each isozyme itself, the relative level of enzyme activity.