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建立了反相HPLC法测定清胃黄连中小檗碱和掌叶防己碱两种有效成分含量的方法，通过对3个批号的测定，平均含量分别为192．57μg／g、95．06μg／g，平均加样回收率为96．5％（RSD＝2．90％）、95．4％（RSD＝3．05％）。
A method for the determination of berberine and palmitrine in Coptis chinensis by reversed-phase HPLC was established. The average content of the three batches was 192.57 μg/g and 95.06 μg/g, respectively. The average recovery was 96.5% (RSD = 2.90%) and 95.4% (RSD = 3.05%).