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利用红外光谱法对正常乳腺组织、良性肿瘤和乳腺癌组织进行了对比研究 .与正常组织的光谱相比 ,癌组织中蛋白质的某些氨基酸残基的νC— O( H) 谱带位置明显向高波数位移 ,表明组织中该基团中的氢键大部分被破坏 .蛋白质分子的氢键化的νNH谱带位置向低波数位移 ,且谱带形状也有所改变 ,说明 NH… O=C氢键化程度增强 .核酸的磷酸二酯基团的 νs,PO- 2 谱带吸收强度明显增强 ,反映癌细胞内 DNA相对含量增加 ;位于 970 cm- 1 附近的νs,PO- 3谱带强度增加 ,提示细胞中磷酸化蛋白含量增加 .而胶原蛋白的特征谱带强度减弱 ,说明其相对含量减少 .研究证明 ,红外光谱可在分子水平上揭示乳腺肿瘤的特征
The normal breast tissues, benign tumors and breast cancer tissues were compared by infrared spectroscopy.Compared with the normal tissues, the νC-O (H) bands of certain amino acid residues in the cancer tissues were obviously High wave number shift, indicating that most of the hydrogen bond in the group of the organization is destroyed.High molecular hydrogen bond νNH band position shift to low wavenumbers, and the band shape has changed, indicating NH ... O = C hydrogen The degree of bonding is enhanced.The absorption intensity of the νs and PO_2 bands in the phosphodiester of the nucleic acid is significantly increased, which indicates that the relative content of DNA in the cancer cells is increased. The intensity of the νs and PO-3 bands located near 970 cm -1 increases , Suggesting that the phosphorylated protein content in cells increased while the intensity of the characteristic bands of collagen decreased, indicating that the relative content decreased.Research has shown that infrared spectroscopy can reveal the characteristics of breast tumors at the molecular level