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目的研究乌头碱、新乌头碱和次乌头碱在不同主产地不同附子炮制品中的含量变化,为附子中药的行业质量标准的建立提供依据。方法采用Alltimate C18色谱柱(250 mm×4.6 mm,5μm);以40 mmol·L-1醋酸铵(氨水调p H=10.00)-乙腈为流动相梯度洗脱;流速1.0 ml·min-1;检测波长240 nm。结果三种单酯型生物碱新乌头碱、乌头碱、次乌头碱均得到良好的分离,回归方程分别为:Y=15.952X-16.118 R2=0.9997;Y=15.906X-2.6445 R2=0.9998;Y=13.218X-23.044 R2=0.9999。三者线性范围是2.5~250μg,2.2~220μg,2.6~260μg。结论该方法的精密度、重复性和稳定性良好,可用于附子炮制品的鉴别和内在质量控制。
Aim To study the changes of contents of aconitine, mesaconitine and hypaconitine in products of different aconites from different main producing areas, and to provide the basis for the establishment of quality standard of aconite Chinese medicine. Methods Alltimate C18 column (250 mm × 4.6 mm, 5 μm) was used. The mobile phase consisted of 40 mmol·L -1 ammonium acetate (p H = 10.00) and acetonitrile (1.0 ml · min -1) Detection wavelength of 240 nm. Results The three monoester alkaloids, mesaconitine, aconitine and hypaconitine were well separated. The regression equations were as follows: Y = 15.952X-16.118 R2 = 0.9997; Y = 15.906X-2.6445 R2 = 0.9998; Y = 13.218X-23.044 R2 = 0.9999. The linear range of the three is 2.5 ~ 250μg, 2.2 ~ 220μg, 2.6 ~ 260μg. Conclusion The method has good precision, repeatability and stability and can be used for the identification and internal quality control of aconite processed products.