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瘦素(leptin)通过动物下丘脑-垂体轴对其生殖活动予以调节,但瘦素通过下丘脑对子宫调节作用仍不清楚.本研究在大鼠侧脑室微量注射瘦素6 h后,采用双向凝胶电泳(2-DE)结合基质辅助激光解吸电离飞行时间质谱(MALDI-TOF-MS)技术分离、鉴定了卵巢摘除给予雌激素的子宫腔液蛋白质表达谱.我们获得了分辨率较高、重复性较好的2-DE图谱;质谱分析结合Swiss Prot蛋白数据库检索,共鉴定了24个差异表达的蛋白质与生理盐水注射(对照)比较,侧脑室微量注射瘦素引起22个蛋白质分子显著上调,2个显著下调.重要的是,在上调的蛋白质分子中包括转铁蛋白、基质金属蛋白酶7、膜联蛋白A1、补体B因子、磷酸甘油酸酯变位酶1、烯醇酶、冠蛋白-1A等15个蛋白质分子,下调的2个蛋白质分子是补体C3、溶解物载体家族4成员3.这些蛋白质分子涉及细胞分化、细胞迁移、细胞凋亡,以及炎症反应等功能.实验结果提示,瘦素可能通过下丘脑-垂体轴参与免疫调节、雌性生殖及炎症反应等,从而对子宫发挥调节作用.本研究将为加深对瘦素调节子宫作用的认识,并为深入研究女性生殖系统疾病提供新的启示.
Leptin regulates its reproductive activities through the hypothalamus-pituitary axis in animals, but the regulation of leptin on the uterus through the hypothalamus remains unclear.In this study, leptin microinjection into the lateral ventricle of rats for 6 h followed by bidirectional (2-DE) combined with matrix-assisted laser desorption / ionization time of flight mass spectrometry (MALDI-TOF-MS) was used to separate and identify the protein expression profile of uterine cavity fluid extracted from estrogen by ovariectomy.We obtained high resolution, Repeatability of 2-DE spectra; Mass spectrometry analysis and Swiss Prot protein database search, a total of 24 differentially expressed proteins were identified compared with saline injection (control), microinjection of leptin into the lateral ventricle caused a significant increase in 22 protein molecules , Two significant downregulation.Important is that among the up-regulated protein molecules including transferrin, matrix metalloproteinase 7, Annexin Al, complement factor B, phosphoglycerate mutase 1, enolase, crown protein -1A and other 15 protein molecules, down two protein molecules complement C3, lysate carrier family 4 members 3. These protein molecules involved in cell differentiation, cell migration, apoptosis, and inflammation And other functions.Experimental results suggest that leptin may regulate the uterus through the hypothalamus-pituitary axis involved in immune regulation, female reproduction and inflammatory response, etc. This study will deepen the understanding of the role of leptin in regulating the uterus, In-depth study of female reproductive system diseases provide new inspiration.