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目的:检测胃癌患者7号染色体长臂微卫星位点的杂合性缺失(lossofheterozygosity,LOH),以初步确定7号染色体长臂上与胃癌相关基因连锁最密切的微卫星多态位点及LOH的临床意义。方法:在70例原发性胃癌中应用多重PCR技术扩增覆盖整个7号染色体长臂的9个微卫星位点(平均遗传距离为10cm),聚丙烯酰胺凝胶电泳分离PCR产物,用GeneScan、Genotyper软件进行分析。结果:9个微卫星位点的LOH均可发生于原发性胃癌,总的LOH频率为34.3%(24/70),其中D7S486和D7S798位点的LOH频率较高,分别为24.0%(12/50)和19.2%(5/26);总的LOH频率随临床分期而显著增高(P=0.046),D7S486位点的LOH频率在淋巴结转移者显著高于无淋巴结转移者(P=0.015)。结论:在7号染色体长臂D7S486和D7S798位点附近,可能存在与胃癌发展相关的抑癌基因。
OBJECTIVE: To detect the loss of heterozygosity (LOH) at the long arm of chromosome 7 in patients with gastric cancer, and to identify the most closely linked microsatellite loci and LOH The clinical significance. Methods: Nine microsatellite loci (average genetic distance was 10 cm) covering the entire long arm of chromosome 7 were amplified by multiplex PCR in 70 cases of primary gastric cancer. The PCR products were separated by polyacrylamide gel electrophoresis and analyzed by GeneScan , Genotyper software for analysis. Results: LOH in all 9 microsatellite loci occurred in primary gastric cancer with a total LOH frequency of 34.3% (24/70). The frequency of LOH was higher in D7S486 and D7S798 sites (24.0%, 12%, respectively) / 50) and 19.2% (5/26) respectively. The total LOH frequency was significantly increased with clinical stage (P = 0.046). The frequency of LOH in D7S486 was significantly higher in patients with lymph node metastasis than those without lymph node metastasis (P = 0.015) . Conclusion: Tumor suppressor genes related to the development of gastric cancer may exist near D7S486 and D7S798 sites on chromosome 7.