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目的探讨苦参碱对淋巴瘤细胞株Raji细胞增殖及Notch信号通路的影响。方法试验分为5组:对照组(0 g/L)和4个苦参碱组(0.5、0.75、1.0、1.5 g/L),分别于苦参碱作用于Raji细胞24、48、72 h后,采用MTT法检测细胞增殖,流式细胞仪检测细胞凋亡及周期分布,RT-PCR法及Western blot法检测Notch1受体及下游靶基因Hes1 m RNA转录及蛋白的表达。结果不同浓度苦参碱均有抑制Raji细胞增殖的作用,且随药物浓度增加及作用时间延长,抑制作用更明显;苦参碱可明显诱导Raji细胞凋亡;经苦参碱1.0 g/L处理48 h后,G1期细胞减少(P<0.05),S期细胞增多(P<0.05),Notch1受体及下游靶基因Hes1 m RNA转录水平及蛋白表达水平均明显下调(P<0.01)。结论苦参碱可抑制淋巴瘤细胞株Raji细胞增殖,且呈浓度-时间依赖性,并诱导细胞凋亡,使细胞周期停留于S期,可能通过直接或间接下调Notch信号通路相关基因表达而实现。
Objective To investigate the effects of matrine on the proliferation and Notch signaling pathway in Raji cells. Methods The experiment was divided into 5 groups: control group (0 g / L) and 4 matrine groups (0.5, 0.75, 1.0 and 1.5 g / L) Cell proliferation was detected by MTT assay. Apoptosis and cell cycle distribution were detected by flow cytometry. The transcription and protein expression of Notch1 receptor and downstream target gene Hes1 m RNA were detected by RT-PCR and Western blot. Results Matrine at different concentrations inhibited the proliferation of Raji cells. With the increase of drug concentration and time, the inhibitory effect was more obvious. Matrine significantly induced the apoptosis of Raji cells. After treatment with matrine 1.0 g / L After 48 h, the number of cells in G1 phase decreased (P <0.05) and the number of S phase cells increased (P <0.05). The transcription level and protein expression of Notch1 receptor and downstream target gene Hes1 m RNA were significantly decreased (P <0.01). Conclusion Matrine can inhibit the proliferation of Raji cells in a time-and dose-dependent manner and induce cell cycle arrest in S phase, possibly by directly or indirectly down-regulating Notch signaling pathway-related gene expression .