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罗斯河病毒(Ross River Virus)是能引起流行性多关节炎的a病毒.本文作者用二氮丙啶(BEI)破坏罗斯河病毒的传染性制成灭活疫苗,在小鼠中评价其免疫效果.作者从急性病人血清中分离出罗斯河病毒,通过在Vero细胞中培养获得大量病毒.离心除去细胞,在上清液中加入2-溴乙胺,37℃水浴,然后加入NaOH提高pH值至8.5～9.0,形成BEI,灭活病毒.最后加入终浓度为10%V/V(0.1M)的硫代硫酸钠终止BEI活性.离心、回收病毒.用无菌盐水重新悬浮病毒颗粒制成BEI灭活疫苗.佐剂灭活疫苗为每ml无菌盐水中含2～300μg灭活病毒和1mg氢氧化铝佐剂.
Ross River Virus is a virus that causes pandemic arthritis.In this study, the authors used Inosine (BEI) to inactivate the infectivity of the Ross River virus inactivated vaccine and evaluated its immunization in mice Results The authors isolated the Ross virus from acute patient serum and obtained a large number of viruses by culturing in Vero cells, centrifuging to remove cells, adding 2-bromoethylamine to the supernatant, 37 ° C water bath, and then adding NaOH to raise the pH To 8.5-9.0 to form a BEI to inactivate the virus, finally BEI activity is terminated by the addition of sodium thiosulfate at a final concentration of 10% V / V (0.1M), centrifugation, recovery of the virus, resuspension of the virus particles with sterile saline BEI inactivated vaccine adjuvant inactivated vaccine per ml sterile saline containing 2 ~ 300μg inactivated virus and 1mg aluminum hydroxide adjuvant.