支原体污染人和动物细胞系是体外组织培养中常见的问题。这种污染不但影响细胞的生长和代谢,还影响细胞的功能,特别是在杂交瘤的制备中,这种影响尤为明显。目前已经建立的几种支原体去除方法,有的会引起细胞突变,有的导致细胞对抗生素耐受或细胞存活力下降。为了解决这些问题,本文作者建立了一种新方法,即将受染细胞与人血单核细胞(BM)衍生的巨噬细胞及混合人免疫球蛋白制剂共同培养,结果证明,此法确能永久地去除支原体。作者将鼠腹腔巨噬细胞(PM)以3.5×10~5/ml的浓度接种到24孔培养板上,24小时后 Mycoplasma contaminating human and animal cell lines is a common problem in tissue culture in vitro. This contamination not only affects cell growth and metabolism, but also affects cell function, especially in hybridoma preparation. Currently has been established several mycoplasma removal methods, some will cause cell mutations, and some lead to antibiotic resistance or cell viability decline. In order to solve these problems, the authors of this article have established a new method of co-culturing infected cells with human blood mononuclear cells (BM) -derived macrophages and mixed human immunoglobulin preparations. The results show that this method can indeed permanently Mycoplasma removal. The murine peritoneal macrophages (PM) were seeded onto 24-well plates at a concentration of 3.5 × 10 ~ 5 / ml. After 24 hours