以成人正常睾丸对照胎儿及成人萎缩睾丸,用琼脂糖凝胶电泳法检测了成人睾丸匀浆中LDH同工酶酶谱。只有成人睾丸匀浆中有一特异同工酶区带-LDHx,介于LDH_3与LDH_4之间,其相对活性为15.1%。LDHx作为精原细胞、精子及精液内LDH同工酶的特异分子形式,它对精子发生及生育能力具有重要意义,因此LDHx的消失或相对活性的降低都将反映睾丸组织生育功能的改变。LDHx的检测也展示了对抗生育研究的前景。 Adult normal testis control fetuses and adult atrophic testes, LDH isozyme zymography in adult testis homogenate was detected by agarose gel electrophoresis. Only adult testis homogenate has a specific isozyme zone-LDHx, between LDH_3 and LDH_4, the relative activity of 15.1%. As a specific molecular form of LDH isozymes in spermatogonia, spermatozoa and seminal fluid, LDHx is important for spermatogenesis and fertility. Therefore, the loss of LDHx or the decrease of relative activity will reflect the change of testicular tissue fertility. The testing of LDHx also shows promise for fighting reproductive research.