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目的研究不同干预方法对饮食诱导的高抵抗素血症大鼠抵抗素表达的影响及其与胰岛素抵抗的关系。方法将80只4周龄SD大鼠随机分为高脂组72只和对照组8只。高脂组给予高脂饲料,对照组给予基础饲料。饲养8周后空腹颈静脉采血检测血清抵抗素水平。选取高脂组中抵抗素高于对照组x+2s的大鼠作为饮食诱导的高抵抗素血症模型,并随机分为罗格列酮组、运动组、单纯调整饮食组、高抵抗素血症对照组,每组8只,分别给予罗格列酮+调整饮食、游泳+调整饮食、单纯调整饮食进行干预,共8周。高抵抗素血症对照组不采取干预措施,继续高脂饮食。干预结束后所有大鼠采血测定血清抵抗素、胰岛素和血糖,计算胰岛素敏感指数,检测脂肪组织抵抗素的表达。结果与高抵抗素血症对照组相比,饮食、药物与运动三种干预均使血清抵抗素降低、脂肪组织抵抗素表达下降,胰岛素敏感指数升高(P均<0.01)。其中,罗格列酮与运动组干预效果最好。结论运动与调整饮食是肥胖伴高抵抗素血症个体早期控制抵抗素与胰岛素抵抗的有效干预措施。
Objective To investigate the effects of different interventions on resistin expression in diet-induced hyperresistrexemia rats and its relationship with insulin resistance. Methods Eighty four-week-old SD rats were randomly divided into hyperlipidemia group (n = 72) and control group (n = 8). High-fat group given high-fat diet, the control group given basic diet. Eight weeks after feeding, fasting jugular vein blood was collected to measure serum resistin levels. The rats with hyperlipidemia in which resistin was higher than that of control group x + 2s were selected as the model of diet-induced hyperlipidemia and were randomly divided into rosiglitazone group, exercise group, diet group, high resistin Control group, each group of 8, respectively, to give rosiglitazone + diet, swimming + diet adjustment, simple adjustment of diet intervention, a total of 8 weeks. High-resistmic control group did not take interventions to continue the high-fat diet. Serum resistin, insulin and blood glucose were measured in all rats after intervention. Insulin sensitivity index was calculated and the expression of resistin in adipose tissue was detected. Results Compared with the hyperhomocysteinemia group, the levels of serum resistin, adiponectin and adiponectin in the three groups were lower than those in the control group (P <0.01). Among them, rosiglitazone and exercise group intervention best. Conclusion Exercise and diet adjustment are effective interventions for early control of resistin and insulin resistance in individuals with obesity and hyperresoninemia.