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建立了HPLC法测定地龙舒腰胶囊中薯蓣皂苷的含量。采用C18色谱柱,以乙腈-水(50∶50)为流动相。结果表明,薯蓣皂苷在7.2～720 g/ml范围内线性关系良好,平均加样回收率为103.4%。以亮氨酸和缬氨酸为对照,采用薄层色谱法鉴别了胶囊中的地龙,结果色谱斑点清晰,阴性无干扰。采用UV法对其中双酯型生物碱进行限度检查。结果 3批胶囊中双酯型生物碱均低于0.15%(以乌头碱计)。
A HPLC method was established for the determination of dioscin in Di Long Shou Ya Capsule. C18 column with acetonitrile-water (50:50) as mobile phase. The results showed that diosgenin showed good linearity in the range of 7.2-720 g / ml with an average recovery of 103.4%. Leucine and valine as controls, using thin layer chromatography to identify the earthworm capsule, the results of chromatographic spots clear, negative non-interference. The use of UV method for double-ester alkaloid limit test. Results The diester alkaloids in 3 batches of capsules were all less than 0.15% (based on aconitine).