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目的探讨脂蛋白脂酶(LPL)基因HindⅢ酶切多态性与维吾尔族2型糖尿病及其体内脂质谱的关系。方法依据病例-同胞对照设计方法,采用全自动生化分析仪等技术测定维吾尔族人群体内脂质谱;应用聚合酶链反应-限制性内切酶长度多态性(PCR-RFLP)方法,对维吾尔族62例2型糖尿病患者、62例糖耐量受损者和124名正常人LPL基因HindⅢ酶切多态性进行分析。结果三组人群的LPL基因的基因型分布和等位基因频率均无统计学意义。H+H+组、H-H-组、H+H-组的TG水平分别为(2.26±1.35)、(1.73±1.31)、(1.80±0.95)mmol/L,三种基因型与体内脂质等指标比较,突变型H+H+组的TG明显大于其他各组。多因素Logistic回归分析显示,TG(P=0.034)和腰围(P=0.001)是2型糖尿病独立危险因素。结论LPL基因HindⅢ酶切多态性与新疆维吾尔族人群2型糖尿病的危险性并无统计学上的相关性,LPL基因突变可能是引起血浆TG水平升高的因素之一。
Objective To investigate the association between Hind Ⅲ polymorphism of lipoprotein lipase (LPL) gene and type 2 diabetes in Uygur and its lipid profile in vivo. Methods According to the case-sibling control design method, the lipid profiles of Uighur population were determined by automatic biochemical analyzer and other methods. The polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) 62 cases of type 2 diabetes mellitus, 62 cases of impaired glucose tolerance and 124 normal subjects LPL gene Hind Ⅲ restriction enzyme digestion polymorphism analysis. Results There was no significant difference in the genotype distribution and allele frequency of LPL gene among the three groups. The levels of TG in H + H + group, HH + group and H + H- group were (2.26 ± 1.35), (1.73 ± 1.31) and (1.80 ± 0.95) mmol / L, In comparison, the TG of mutant H + H + group was significantly higher than that of other groups. Multivariate Logistic regression analysis showed that TG (P = 0.034) and waist circumference (P = 0.001) were independent risk factors for type 2 diabetes. Conclusion There is no statistical correlation between the HPLIII polymorphism of LPL gene and the risk of type 2 diabetes in Xinjiang Uygur population. LPL gene mutation may be one of the factors that cause the increase of plasma TG level.