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目的观察补肾生精丸对腺嘌呤所致生精细胞损伤模型大鼠生精细胞凋亡的影响及凋亡相关基因的调控情况。方法SD大鼠40只随机分为4组:正常组、模型组、中药治疗组(补肾生精丸组)、中药对照组(五子衍宗丸组)。以腺嘌呤灌胃诱发生精细胞损伤肾阳虚模型,用TUNEL法检测各组大鼠睾丸组织生精细胞的凋亡情况;用免疫组化的方法检测Bcl-2、Bax、Fas及FasL的表达。结果补肾生精丸能够抑制大鼠睾丸曲细精管生精细胞的凋亡,上调抑制凋亡基因Bcl-2的表达,下调促凋亡基因Bax、Fas、FasL的表达,与模型组比较有统计学意义(P<0.01)。结论补肾生精丸对腺嘌呤致生精细胞损伤模型大鼠生精细胞的凋亡有一定的抑制作用,其作用可能与该药调控Bcl-2、Bax、Fas、FasL的表达有关。
Objective To observe the effects of Bushen Shengjing Pills on the apoptosis of spermatogenic cells in adenycine-induced spermatogenic cell damage model and the regulation of apoptosis-related genes. Methods Forty SD rats were randomly divided into 4 groups: normal group, model group, TCM treatment group (Bushen Shengjing Pill group) and TCM control group (Wuzi Yanzong pill group). Adenine was intragastrically induced spermatogenic cell injury model of kidney yang, TUNEL method was used to detect the apoptosis of spermatogenic cells in testis tissue of rats in each group. The expressions of Bcl-2, Bax, Fas and FasL were detected by immunohistochemistry expression. Results Bushen Sheng Jing Wan inhibited the apoptosis of rat seminiferous tubule germ cells, up-regulated the expression of Bcl-2, decreased the expression of pro-apoptotic genes Bax, Fas and FasL, compared with the model group Statistical significance (P <0.01). Conclusion Bushen Sheng Jing Wan can inhibit the apoptosis of spermatogenic cells of adenyloid-induced spermatogenic cells in rats, which may be related to the regulation of Bcl-2, Bax, Fas and FasL expression.