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目的:探讨体外缺氧条件下骨髓间充质干细胞(MSCs)凋亡膜微粒(MSC-MPs)对心脏成纤维细胞(CFs)增殖、迁移和胶原合成的影响。方法:提取SD大鼠MSCs,在缺氧低营养条件下培养,收集上清液并提取MSC-MPs,用MSC-MPs刺激大鼠CFs,用CCK-8和划痕实验分别检测CFs增殖和迁移,qRT-PCR检测CFs中I型胶原、II型胶原、血管平滑肌肌动蛋白(α-SMA)、波型蛋白和纤连蛋白的合成情况。结果:CFs被MSC-MPs刺激后,与对照组比较,其增殖能力明显增加(P<0.05),迁移能力无明显变化(P>0.05),I型胶原、I型胶原、波型蛋白和纤连蛋白合成增加(均P<0.05)。结论:体外缺氧条件下MSC-MPs可促进CFs的增殖和胶原合成能力,而对CFs的迁移能力无明显影响。
Objective: To investigate the effects of MSC-MPs on the proliferation, migration and collagen synthesis of cardiac fibroblasts (CFs) under hypoxia. Methods: MSCs of SD rats were isolated and cultured in hypoxia and low nutrition condition. Supernatants were collected and MSC-MPs were harvested. CFs were stimulated with MSC-MPs. The proliferation and migration of CFs were detected by CCK-8 and scratch assay The synthesis of type I collagen, type II collagen, vascular smooth muscle actin (α-SMA), vimentin and fibronectin in CFs was detected by qRT-PCR. RESULTS: After stimulation with MSC-MPs, the proliferation of CFs was significantly increased (P <0.05) and the migration ability was not significantly different (P> 0.05). Collagen type I, type I collagen, Increased protein synthesis (both P <0.05). CONCLUSION: MSC-MPs can promote the proliferation and collagen synthesis of CFs under hypoxic conditions, but have no significant effect on the migration ability of CFs.