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应用Ames试验对碳素粉尘致突变检测前处理方法进行研究。比较索氏萃取法(Sexheltextraction及超声振动萃取法和不同萃取溶剂-环已烷、二氯甲烷及甲醇的萃取效果。经统计处理,选出萃取方法与溶剂的最佳搭配的前处理方法,移码突变检测-TA98(±S9),选用索氏萃取法、二氯甲烷溶剂。碱基置换致突变检测-TA100(±S9)选用超声振动萃取法、二氯甲烷溶剂。
Ames test was used to study the pretreatment method of carbon dust induced mutation. Comparison of Soxhlet extraction (ultrasonic extraction method and ultrasonic vibration extraction and extraction solvent - cyclohexane, methylene chloride and methanol extraction effect.According to statistical processing, choose the best combination of extraction method and solvent pretreatment method, shift Mutation detection-TA98 (± S9), the choice of Soxhlet extraction, methylene chloride solvent base mutation detection mutation-TA100 (± S9) selection of ultrasonic vibration extraction, methylene chloride solvent.