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(一)磷酰胺类化合物在酸性及碱性溶液中都不稳定。在所试过的化合物中,以磷酰对氯苯胺最稳定,但它的溶解度较小。磷酰参羧基苯胺和磷酰苯甲胺的自行分解速度甚大,不过宜用为底物。 (二)牛脾部分提纯制剂在 pH5.0对于磷酰胺类化合物的活力比在 pH9.0的高。而对于不同底物的水解速度与亲和力都不相同,显示磷酸氨基酶活力与底物的结构有密切关系。在分解产物中,胺类对于该酶的活力无大影响,而磷酸根离子则有较为显著的抑制作用。(三)大鼠组织提取液对于结构相似的底物的活力之比也相似,而对于结构不同的底物,则活力之比也不相同,显示有不同磷酸氨基酶存在的可能。(四)各组织中磷酸氨基酶的含量不同,以脾脏的含量最高。(五)肌肉组织中合有磷酸氨基酶的活力,它的研究可能对于肌肉中磷酸肌酸代谢的了解有所帮助。
(A) Phosphoramidon compounds are unstable in both acidic and alkaline solutions. Of the compounds tested, phosphoryl p-chloroaniline was the most stable, but its solubility was lower. Phosphoryl-reference carboxyaniline and phosphoryl benzylamine self-decomposition rate is great, but should be used as a substrate. (B) part of the purified preparation of cattle spleen at pH5.0 for the activity of phosphoramidates than at pH9.0 high. The hydrolysis rate and affinity for different substrates are not the same, indicating that the phosphorylation of amino enzyme activity and substrate structure are closely related. In the decomposition products, amines have no significant effect on the enzyme activity, while phosphate ions have a more significant inhibitory effect. (3) The ratio of viability of rat tissue extracts to similar substrates is also similar, whereas the ratio of viability is also different for substrates with different structures, indicating the possibility of different phosphoamidases. (D) different levels of phospho-amino enzyme in various organizations to the highest content of the spleen. (E) muscular tissue combined with phosphorylation of amino enzyme activity, its research may be muscle creatine phosphorylation of metabolic help.