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目的 探讨三氧化二砷 (As2 O3 )对人肺癌的潜在性治疗作用及可能的机制。方法 选用人肺癌细胞株GLC 82 ,运用细胞培养法、MTT法、流式细胞术 (FCM)检测细胞生长曲线、细胞增殖、细胞周期和细胞凋亡。结果 三氧化二砷可明显抑制GLC 82细胞的增殖 ,其抑制作用具有时—效和量—效关系。当 4.0 μmol/L三氧化二砷处理GLC 82细胞 96小时 ,增殖抑制率达 81.0 5 %。FCM检测肿瘤细胞DNA含量 ,观察到三氧化二砷使GLC 82细胞周期阻滞于G2 /M期 ,细胞周期进程变慢 ,同时出现剂量依赖性亚G1峰。结论 三氧化二砷能有效地抑制人肺癌细胞株GLC 82的增殖 ,其可能的机制与三氧化二砷使细胞阻滞于G2 /M期并诱导其凋亡有关。
Objective To explore the potential therapeutic effects and possible mechanisms of arsenic trioxide (As2O3) on human lung cancer. Methods Human lung cancer cell line GLC 82 was used. Cell growth curve, cell proliferation, cell cycle and apoptosis were detected by cell culture method, MTT assay and flow cytometry (FCM). Results Arsenic trioxide can significantly inhibit the proliferation of GLC 82 cells. The inhibitory effect of arsenic trioxide has a time-effect and dose-effect relationship. When GLC 82 cells were treated with 4.0 μmol/L arsenic trioxide for 96 hours, the proliferation inhibition rate was 81.05%. The DNA content of tumor cells was detected by FCM. It was observed that arsenic trioxide arrested the cell cycle of GLC 82 at G2 /M phase, and the cell cycle progressed slowly, and a dose-dependent sub-G1 peak appeared. Conclusion Arsenic trioxide can effectively inhibit the proliferation of human lung cancer cell line GLC 82. Its possible mechanism is related to arsenic trioxide arresting cells in G2 /M phase and inducing apoptosis.