用高压液相色谱法测定人体血浆内阿米替林及其代谢产物去甲替林的浓度。以国产硅胶YQG—80(7μm)为固定相,甲醇—水极性流动相,三甲丙咪嗪及普罗替林为双内标物,紫外检测波长240nm,阿米替林及去甲替林最低检出限量分别为2ng、5ng,最低检出浓度为4ng/ml、10ng/ml(血浆),回收率分别为>94％,>97％。方法简便、灵敏度高、专一性强。 Determination of amitriptyline and its metabolite nortritin in human plasma by high pressure liquid chromatography. Using domestic silica gel YQG-80 (7μm) as stationary phase, methanol-water polar mobile phase, trimethoprim and protriptyline as double internal standard, the detection wavelength was 240nm, the lowest was amitriptyline and nortriptyline The limit of detection was 2ng and 5ng respectively. The lowest detectable concentration was 4ng / ml and 10ng / ml (plasma). The recoveries were> 94% and> 97% respectively. The method is simple, high sensitivity and specificity.