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目的:建立HPLC法测定银翘解毒片中绿原酸的含量。方法:采用AE.Lichrom C_(18)柱(150mm×4.6mm,5μm);以乙腈:0.4%磷酸溶液(10∶90)为流动相;检测波长为327nm;流速为1.0ml·min~(-1);室温操作。结果:绿原酸在10.16～101.60μg·nl~(-1)的浓度范围内呈线性关系,r=0.999 8,平均回收率为98.8%,RSD为1.0%。结论:该法简便、准确可靠,可用于该制剂的质量控制。
Objective: To establish HPLC method for determination of chlorogenic acid in Yinqiao Jiedu Tablets. Methods: AE. Lichrom C 18 column (150mm×4.6mm, 5μm) was used; acetonitrile: 0.4% phosphoric acid solution (10∶90) was used as mobile phase; detection wavelength was 327nm; flow rate was 1.0ml·min~(- 1); operating at room temperature. RESULTS: The linearity of chlorogenic acid was in the range of 10.16-101.60 μg·nl-1. r=0.999 8. The average recovery was 98.8% and the RSD was 1.0%. Conclusion: This method is simple, accurate and reliable and can be used for the quality control of the preparation.