论文部分内容阅读
目的评价反向蛋白质微点阵方法是否能用于怀孕女性TORCH感染的检测。方法建立检测TORCH感染的反向蛋白质微点阵方法,比较反向蛋白质微点阵和酶联免疫吸附试验(ELISA)方法检测2 000份怀孕女性的新鲜血清TORCH感染的阳性符合率,评价反向蛋白质微点阵检测TORCH的可行性。结果建立的反向蛋白质微点阵方法与ELISA方法对TORCH感染检测的阳性符合率分别为100.0%、91.1%、97.2%、91.3%和93.0%,通过蛋白芯片法与ELISA法各项指标检测结果均具有较好的一致性(P>0.05),但反向蛋白质微点阵对风疹病毒、巨细胞病毒和疱疹病毒的检测率高于相对应的ELISA方法。结论反向蛋白微点阵法检测TORCH感染,具有简便、快速,敏感性较高和特异性强等优点,是临床优生优育辅助诊断的有效方法,未来值得推广使用。
Objective To evaluate whether the reverse protein microarray method can be used to detect TORCH infection in pregnant women. Methods The reverse protein microarray method for detecting TORCH infection was established. The positive coincidence rate of fresh serum TORCH infection in 2 000 pregnant women was compared by reverse protein microarray and enzyme-linked immunosorbent assay (ELISA) Feasibility of protein microarray detection of TORCH. Results The positive coincidence rates of reverse protein microarray method and ELISA method for detection of TORCH infection were 100.0%, 91.1%, 97.2%, 91.3% and 93.0%, respectively. The results of protein chip assay and ELISA assay (P> 0.05). However, the detection rate of rubella virus, cytomegalovirus and herpesvirus by inverted protein microarray was higher than that of ELISA. Conclusion The reverse protein microarray detection of TORCH infection has the advantages of simple, rapid, sensitive and specific. It is an effective method to assist the diagnosis of prenatal and postnatal care. It is worth promoting in the future.