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郎格罕氏岛的移植1.郎氏岛的分离:分离方法分机械性细切法和胶原酶消化法两种。两者主要区别是:机械性法获得的郎氏岛不受药物影响,但必须有熟练技术,一次获大量的郎氏岛是不可能的。胶原酶法的技术容易掌握,从一只大鼠中一次可获得郎氏岛200—300个,可是排除胶原酶损害的郎氏岛是困难的。因此,用最少量胶原酶在最短的时间内完成消化,可获得成活率较高的郎氏岛。首先,确定胶原酶的浓度,消化时间,温度和振荡程度等问题。作者等确定胶原
Langagen Island transplantation 1. Lang Island separation: separation method sub-mechanical fine cut method and collagenase digestion two. The main difference between the two is: Lang’s island obtained by mechanical method is not affected by drugs, but it must have the skill to obtain a large amount of Lang Island at one time is impossible. The technique of collagenase is easy to grasp. 200-300 Lang’s Island can be obtained from one rat at a time, but it is difficult to exclude Lang’s Island from collagenase damage. Therefore, with a minimum amount of collagenase in the shortest possible time to complete digestion, we can get a higher survival rate Lang Island. First, determine the collagenase concentration, digestion time, temperature and oscillation degree and other issues. The authors determine the collagen