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为明确马铃薯早疫病病菌产生细胞壁降解酶的条件,采用液体培养基培养病菌。结果表明,病菌在改良的Marcus液体培养基中能产生多聚半乳糖醛酸酶(PG)、果胶甲基半乳糖醛酸酶(PMG)、纤维二糖水解酶(Cx)和β-葡萄糖苷酶(βG)细胞壁降解酶。其中,果胶酶(PG、PMG)的活性较高,纤维素酶(Cx、βG)的活性较低。病菌产生果胶酶的适宜条件为培养液初始p H为4.0,30℃持续振荡培养4 d,纤维素酶初始p H为5.0,25℃持续振荡6 d。
In order to clarify the condition of cell-wall degrading enzyme produced by potato asphodeloides, the liquid medium was used to cultivate the bacteria. The results showed that the bacteria could produce polygalacturonase (PG), pectin methyl galacturonase (PMG), cellobiohydrolase (Cx) and β-glucose in a modified Marcus liquid medium Glycosidase (βG) Cell wall degrading enzyme. Among them, pectinase (PG, PMG) activity is higher, cellulase (Cx, βG) activity is low. The optimal conditions for the production of pectinase by the bacteria were as follows: the initial pH of the culture solution was 4.0, the shaking culture was continued at 30 ° C for 4 days, the initial pH of the cellulase was 5.0, and the oscillation was continued at 25 ° C for 6 days.