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经常发现人体肿瘤经药物治疗消退后又复发,人体对许多药物都具有抗药性。了解这种现象对研制更有效的化疗有重要意义。Ger-lach等(Nature,324卷1986年12月)报告,P-糖蛋白和hlyB基因产物的氨基酸序列非常相似。P-糖蛋白是相对分子量为170K的膜蛋白,P-糖蛋白过多蓄积可导致对多种药物有抗药性。hlyB基因产物为66K的膜蛋白,它调节从大肠杆菌主动排出的溶血素蛋白。这种相似性足以表明P-糖蛋白的功能。另外两个研究组最近报告发现了小鼠和人细胞P-糖蛋白序列中完全互补的DNA(cNDA),其结果与Gerlach等人所获得的结果非常相似。
It is often found that human tumors relapse after drug treatment subsides and the body is resistant to many drugs. Understanding this phenomenon has important implications for the development of more effective chemotherapy. Ger-lach et al. (Nature, volume 324, December 1986) reported that the amino acid sequences of the P-glycoprotein and hlyB gene products are very similar. P-glycoprotein is a membrane protein with a relative molecular weight of 170K, and excessive accumulation of P-glycoprotein can lead to resistance to various drugs. The hlyB gene product is a 66K membrane protein that regulates the active excretion of hemolysin protein from E. coli. This similarity is sufficient to indicate the function of P-glycoprotein. Two other groups recently reported finding completely complementary DNA (cNDA) in mouse and human cell P-glycoprotein sequences, and the results are very similar to those obtained by Gerlach et al.