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近来有人将纯化抗原应用于华支睾吸虫病的诊断,取得了较满意的结果。我们进一步应用硫酸铵分段沉淀华支睾吸虫不同抗原,进行酶联免疫吸附试验及皮内试验,以评价其应用阶值,报道如如下。一、材料与方法(一)华支睾吸虫抗原1.粗提抗原:自人工感染华支睾吸虫囊蚴的猫肝脏中取华支睾吸虫成虫200余条。以生理盐水洗
Recently, some people apply purified antigen to the diagnosis of Clonorchiasis, and have achieved satisfactory results. We further segmented precipitation of different antigen of Clonorchis sinensis using ammonium sulfate, enzyme-linked immunosorbent assay and intradermal test to evaluate its application order, reported as follows. First, the material and methods (A) Clonorchis sinensis antigen 1. Crude extract antigens: Since artificial infection of Clonorchis sinensis metacercarid cat liver clonorchiasis adult more than 200 articles. Wash with saline