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目的建立C群流行性脑膜炎球菌多糖50 L罐分批发酵动力学模型,为实现对C群流行性脑膜炎球菌多糖发酵过程的工艺控制、优化及其放大提供理论依据。方法利用分批发酵的数据,以Logistic方程建立菌体生长动力学模型,以Leudedeking-Piret微分方程建立产物合成动力学模型,以底物消耗的物料平衡方程建立底物消耗的动力学模型,并应用Origin软件进行模型拟合,获得3个模型的参数。结果菌体的最大比生长速率(μmax)为0.731 2 h-1,最大菌体浓度(Xmax)为3.949 5 g/L,与菌体生长速率关联的产物合成常数(a)为0.004 97 g/(L·h),与菌体浓度关联的产物合成浓度(β)为0.015 44 g/(L·h),菌体对底物的得率(Yx)为1.905 2 g/g,产物对底物的得率(Yp)为0.130 1 g/g,菌体的维持因子(m)为1.070 1。结论建立的动力学模型能较好地反映C群流行性脑膜炎球菌多糖分批发酵过程中菌体生长、C群多糖合成和基质消耗的的变化规律。
OBJECTIVE: To establish a batch fermentation kinetic model of epidemic meningococcal polysaccharide (C group) in a 50 L tank, and to provide a theoretical basis for the control, optimization and amplification of the epidemic meningococcal polysaccharide fermentation in group C. Methods Based on batch fermentation data, kinetic model of mycelia growth was established by Logistic equation and kinetic model of product synthesis was established by Leudedeking-Piret differential equation. The kinetic model of substrate consumption was established by the material balance equation of substrate consumption. Apply Origin software for model fitting, get the parameters of the three models. Results The maximum specific growth rate (μmax) was 0.731 2 h-1 and the maximum cell concentration (Xmax) was 3.949 5 g / L. The synthetic constant (a) correlated with the cell growth rate was 0.004 97 g / (L · h). The product concentration (β) was 0.015 44 g / (L · h) and the yield of substrate to substrate (Yx) was 1.905 2 g / g. The yield of the material (Yp) was 0.130 1 g / g, and the cell maintenance factor (m) was 1.070 1. Conclusions The established kinetic model can better reflect the variation of polysaccharide synthesis and matrix consumption during the batch fermentation of meningococcal serogroup C in group C.