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目的通过较高剂量补充维生素Ｅ、Ｃ和β－胡萝卜素，观察三种维生素对淋巴细胞损伤的影响。方法选取５０～５９岁健康男性随机分为实验组和对照组，每组５０人，实验组每人每天服用β－胡萝卜素２５ｍｇ、维生素Ｃ１００ｍｇ，维生素Ｅ２８０ｍｇ，实验期为２０周，采集实验组和对照组的全血分离淋巴细胞。ＤＮＡ损伤程度的分析采用“彗星”电泳技术分析；血清中维生素Ｃ、α－生育酚和β－胡萝卜素的测定均采用高效液相色谱法分析。结果外周血淋巴细胞ＤＮＡ的损伤程度分析发现ＤＮＡ自发损伤率在实验组和对照组人群中均较低，ＤＮＡ损伤率分别为６．１％和６．８％，两组间差异无明显性；但是当实验组和对照组人群外周血淋巴细胞经Ｈ２Ｏ２处理后，对照组人群在３０μｍｏｌ／Ｌ、１００μｍｏｌ／Ｌ、３００μｍｏｌ／ＬＨ２Ｏ２剂量组细胞ＤＮＡ损伤率逐渐升高，分别达到３６．１４％、５９．４５％和６９．６２％，并明显高于实验组Ｈ２Ｏ２相应处理剂量细胞ＤＮＡ损伤率。结论通过补充维生素Ｅ、Ｃ和β－胡萝卜素，可以有效地降低Ｈ２Ｏ２所致的ＤＮＡ损伤
Objective To investigate the effects of three vitamins on lymphocyte injury by vitamin E, C and β-carotene supplementation at higher doses. Methods Healthy male aged 50-59 years were randomly divided into experimental group and control group with 50 in each group. The experimental group took 25 mg of β-carotene, 100 mg of vitamin C1 and 280 mg of vitamin E2 daily for 20 weeks. The experimental group and The control group’s whole blood isolated lymphocytes. Analysis of the degree of DNA damage using “comet” electrophoresis technology analysis; determination of serum vitamin C, α-tocopherol and β-carotene were analyzed by high performance liquid chromatography. Results The degree of DNA damage in peripheral blood lymphocytes was found to be lower in spontaneous DNA damage group than in experimental group and control group. The rates of DNA damage were 6.1% and 6.8%, respectively. There was no significant difference between the two groups. However, when the peripheral blood lymphocytes of experimental group and control group were treated with H2O2, the DNA damage rate of control group was increased to 36.14%, 59%, 59% respectively at 30μmol / L, 100μmol / L, 300μmol / .45% and 69.62%, respectively, which was significantly higher than that of experimental group treated with H2O2. Conclusions By adding vitamin E, C and β-carotene, DNA damage caused by H2O2 can be effectively reduced