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目的:观察防疤烧伤膏对大鼠伤口愈合过程中 TGFβ_1,mRNA 表达及蛋白含量的影响。方法:采用大鼠皮肤切除致伤模型,创面外用防疤烧伤膏,并采用碱性成纤维细胞生长因子(basic fibroblast growth,bFGF)和空白处理作为阳性和阴性对照。伤后3、7、14d 取创面组织,经10%中性福尔马林固定,石蜡包埋,切片后采用免疫组化和原位杂交的方法检测伤口组织中 TGFβ_1mRNA 和其蛋白含量的变化。结果:与空白对照组相比,防疤烧伤膏处理后的创面组织细胞内 TGFβ_1的 mRNA 和蛋白含量明显减少。结论:防疤烧伤膏在促进创面愈合的同时,能通过抑制创面组织细胞中 TGFβ_1的基因表达和蛋白含量的升高,因此,可能使伤口愈合后的瘢痕形成减少。
OBJECTIVE: To observe the effect of Zhibi burn ointment on TGFβ_1 mRNA expression and protein content in wound healing process in rats. METHODS: Rat skin injury model was used to remove wounds. External application of topical burn cream was performed. Basic fibroblast growth (bFGF) and blank treatment were used as positive and negative controls. The wound tissues were taken 3, 7, and 14 days after injury, fixed in 10% neutral formalin and embedded in paraffin. After sliced, immunohistochemistry and in situ hybridization were used to detect changes in TGFβ_1 mRNA and protein content in the wound tissue. RESULTS: Compared with the blank control group, the mRNA and protein content of TGFβ_1 in the wound tissue after treatment with Fangqin Burn Ointment was significantly reduced. Conclusion: The anti-caries burn ointment can promote the healing of wounds and at the same time, it can inhibit the expression of TGFβ_1 gene in the wound tissue and increase the protein content. Therefore, it may reduce the scar formation after wound healing.