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以玉米纹枯病菌菌丝为材料,利用In-Fusion SMARTer TM c DNA Lib Construction Kit构建玉米纹枯病菌全长c DNA文库。文库质量鉴定结果表明,文库滴度为1.2×106pfu·m L-1,重组率为99.2%,插入片段平均长度大于1.0 kb。随机挑取400个白色克隆进行测序,共获得329个高质量EST序列,经聚类拼接后得到250条unique EST,包括36条contigs和214条singletons。在Gen Bank进行Blastn与Blastx同源比对,有227条EST与已知核酸或蛋白有不同程度的同源性,占全部EST的90.8%,其余23条无任何同源性,占9.2%。
The full-length c DNA library of Rhizoctonia solani was constructed by using the In-Fusion SMARTer ™ c DNA Lib Construction Kit with Rhizoctonia solani as the material. The results of library quality identification showed that the titer of the library was 1.2 × 106 pfu · m L-1, the recombination rate was 99.2%, and the average length of the insert was more than 1.0 kb. A total of 400 white clones were randomly selected for sequencing. A total of 329 high quality EST sequences were obtained. After clustering, 250 unique ESTs were obtained, including 36 contigs and 214 singletons. Blastn was homologously aligned with Blastx in Gen Bank. There were 227 ESTs with different degrees of homology to known nucleic acids or proteins, accounting for 90.8% of all ESTs. The other 23 strains shared no homology, accounting for 9.2%.