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Three new cucurbitacins were isolated from the BuOH extract of the rhizomes of Hemsleya lijiangensis. Their structures were elucidated as 23, 24 -dihydro cucurbitacin F-16, 25-diacetate(1), 23,24-dihydro cucurbitacin F-16, 25-diacetate-2-O-β-D -glucopyran