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目的探讨大花红景天水提物(aqueous extract of Rhodiola crenulata,RCE)对二甲基甲酰胺(N,N-dimethylformamide,DMF)致小鼠肝损伤的保护作用及其机制。方法 ICR小鼠按体重随机分为6组:对照组、RCE组(400 mg/kg RCE)、DMF组(1500 mg/kg DMF)、低剂量联合处理组(100 mg/kg RCE+1500 mg/kg DMF)、中剂量联合处理组(200 mg/kg RCE+1500 mg/kg DMF)和高剂量联合处理组(400 mg/kg RCE+1500 mg/kg DMF)。对照组和DMF组每天灌胃双蒸水,RCE组以400 mg/kg RCE灌胃,联合处理组分别先以100、200及400 mg/kg的RCE灌胃,1次/d,共5 d。DMF组及联合处理组于最后一次灌胃30 min后腹腔注射1500 mg/kg DMF生理盐水溶液,对照组及RCE组注射同体积生理盐水。48 h后处死动物,测定血清ALT、AST、LDH活性和总胆汁酸(TBA)含量,肝MDA、SOD、还原型谷胱甘肽(GSH)、氧化型谷胱甘肽(GSSG)和8-羟基脱氧鸟苷(8-OHdG)水平。结果与对照组比较,DMF组血清ALT、AST、LDH活性、血清TBA及肝MDA含量明显升高(P<0.05),肝SOD活力降低(P<0.05),肝8-OHdG含量升高。与DMF组比较,各剂量联合处理组血清ALT和AST活性、TBA含量均降低(P<0.05),中、高剂量联合处理组血清LDH活性和肝MDA含量降低(P<0.05),低剂量联合处理组肝SOD活力升高(P<0.05),各剂量联合处理组肝8-OHdG含量降低。结论大花红景天水提物对DMF所致小鼠肝损伤有保护作用,可能与大花红景天的抗氧化作用有关。
Objective To investigate the protective effect of aqueous extract of Rhodiola crenulata (RCE) on liver injury induced by dimethylformamide (DMF) in mice and its mechanism. Methods ICR mice were randomly divided into 6 groups: control group, RCE group (400 mg / kg RCE), DMF group (1500 mg / kg DMF), low dose combined treatment group (100 mg / kg DMF), medium dose combination treatment group (200 mg / kg RCE + 1500 mg / kg DMF) and high dose combination treatment group (400 mg / kg RCE + 1500 mg / kg DMF). The rats in control group and DMF group were dosed with double distilled water every day. The rats in RCE group were intragastrically infused with RCE at 400 mg / kg, and the combined treatment group was infused with RCE at 100, 200 and 400 mg / kg respectively for 1 d . The rats in DMF group and combined treatment group were given intraperitoneal injection of 1500 mg / kg DMF physiological saline solution 30 min after the last intragastric administration, while the control group and RCE group were injected with the same volume of normal saline. After 48 h, the animals were sacrificed and the activities of serum ALT, AST, LDH and total bile acids (TBA) were determined. The contents of MDA, SOD, GSH, GSSG and 8- Hydroxy-deoxyguanosine (8-OHdG) levels. Results Compared with the control group, serum ALT, AST, LDH activities, serum TBA and liver MDA levels were significantly increased (P <0.05), liver SOD activity was decreased (P <0.05), and liver 8-OHdG content was increased. Compared with DMF group, serum ALT and AST activities and TBA contents decreased (P <0.05), and LDH activity and MDA content decreased (P <0.05) in medium and high dose combined treatment groups, The activity of hepatic SOD in treatment group was increased (P <0.05), and the content of hepatic 8-OHdG in each treatment group was decreased. Conclusion The aqueous extract of Rhodiola rosea has a protective effect on hepatic injury induced by DMF in mice, which may be related to the antioxidant effect of Rhodiola rosea.