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我们在LAK细胞的培养条件中加入5～10μg/ml的PHA,建立了LPAK细胞(Lymphokine and PHA Activated killer cell)的培养方法,并采取用少量血分离淋巴细胞,较长时间扩增培养的方式,使LAK细胞的临床应用在一般实验室条件下更易开展。据我们的实验结果,加PHA的意义在于促进LAK细胞的
We added 5 to 10 μg/ml of PHA to the culture condition of LAK cells to establish a method for culturing LPAK cells (Lymphokine and PHA Activated killer cells) and adopt a method of separating lymphocytes with a small amount of blood for a longer period of time. The clinical application of LAK cells is easier to perform under general laboratory conditions. According to our experimental results, the significance of adding PHA is to promote LAK cells.